Site-directed mutagenesis facilitates the experimental validation of gene function and can speed up plant breeding by producing new biodiversity or by reproducing previously known gene variants in ...

Site-directed mutagenesis (SDM) has been an important feature of the research landscape since its introduction in 1995. Continuing improvements to Pfu DNA polymerase have led to enhancements in the ...

Alanine (Ala) scanning is a widely used mutagenesis approach in which residues in a target protein are systematically substituted for alanine at selected positions by site-directed mutagenesis, ...

In a recent study posted to the bioRxiv* preprint server, a team of researchers investigated the change in the stability, antigenicity, and protein expression of the severe acute respiratory syndrome ...

Protein engineering is a powerful biotechnological process that focuses on creating new enzymes or proteins and improving the functions of existing ones by manipulating their natural macromolecular ...

A new study (Budhagatapalli et al., 2020), which has recently been published in the Plant Biotechnology Journal, demonstrates how site-directed mutagenesis can be achieved in virtually any wheat ...